It is only 2 to 3 days to use the PCR method to detect the botulinum neurotoxin gene to identify Clostridium botulinum. Especially in the case that the primary CDC can not purchase the test animals, the advantages of rapid, simple and specific PCR can be used as an auxiliary detection means and a valuable indicator for botulism.
P. Fach (1993) reported that PCR can be used to detect Clostridium botulinum type A strains in food samples. Zhao Jin, Guo Zongqi, Yang Xiaorong, etc. (2006) reported that the pathogens of botulism food poisoning can be detected by PCR. A pair of synthetic oligonucleotide primers were used to amplify a 264 bp DNA fragment of the botulinum neurotoxin genes of type A, B, C, D, E, F and G, and two foods The enzymatic and toxic culture solution of the poisoned sample and the isolated strain were tested, and the botulinum botulinum was detected from the food poisoning sample. This proves that the PCR method can quickly and accurately identify pathogens in botulinum food poisoning samples.
PCR diagnosis also has the following problems. If only Clostridium botulinum can be detected, only the toxins in the poisoned foods and no Clostridium botulinum are present, the detection of PCR has no diagnostic value; secondly, according to the determination of botulism food poisoning: WS/T83-1996 The PCR diagnostic method and treatment principles for Clostridium food poisoning did not include the PCR method. Therefore, in practice, the PCR method can not replace GB/T4789.12-2003, and can only be used as a valuable auxiliary detection method.
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