Why are broilers dead?

Recently, a farmer in Dongxuegou Village, Tangtang County, purchased 1,500 broilers. They developed symptoms at 3 days of age and became sick on the 7th day of life, killing more than a dozen a day. He came to the veterinary clinic to diagnose chicken colibacillosis with sodium selenite and vitamin E deficiency. The relevant situation is reported as follows:

E. coli disease in broiler chickens is a common disease and chicks at 2-6 weeks of age are the most susceptible. Poor ventilation, over-intensive feeding, high humidity, and environmental pollution often lead to increased morbidity. Broilers grow fast. The lack of sodium selenite and vitamin E in the feed or the absorption of selenium and vitamin E can easily lead to deficiency.

1. Clinical symptoms. On July 6, the farmers purchased 1,500 Avian Broiler stocks. When purchased, they were in good spirits. They were fed and immunized as usual. Diarrhea was found on the 3rd day. Adding ofloxacin to large groups of drinking water had little effect. On the 5th day of age, some dogs suffered from dog sitting and gasping, followed by sporadic deaths. The main manifestations of the chickens were reduced feeding intake, or even abolished. The spirits were stagnant, with both wings drooping, feathers unkempt, mouth stretched, panting, pulling yellow and white dilute feces, and abdomen bruising. The compound was mixed with vitamin B and worsened by the age of 7 days, with more than 10 deaths per day.

2. The necropsy changes. Through the necropsy of 12 dead chickens, the infected chickens had subcutaneous edema of the abdomen, which made the legs diverge outwards. The edema was colored green. When the scissors were cut, a slightly viscous blue-green liquid flowed out. Most of the pericardial effusions were visible, and the heart expanded. The liver is swollen with yellow cellulose protein membranes attached to the surface; there are many fibrous exudates in the abdominal cavity, mesenteric adhesions, and ascites. There were scattered bleeding spots on the surface of individual cerebellum; there were no obvious lesions in other organs.

3. Laboratory diagnosis. 1 bacterial culture. Liver and peritoneal tissues of dead chickens were aseptically collected, inoculated on an agar medium, and incubated in a 37° C. incubator for 24 hours. Normal, a bulging, smooth, and gray-white colonies were observed on the agar medium, and MacConkey culture medium was observed. The colonies are red. Colony smears were picked on a sterile inoculation loop. Microscopic examination after gram staining revealed gram-negative, medium-sized bacilli with dull ends. 2 drug sensitivity test. It was found to be highly sensitive to drugs Conic, ciprofloxacin hydrochloride, enteroline, moderately sensitive to amoxicillin and erythromycin, and low sensitive to penicillin and ofloxacin. 3 Biochemical tests were identified as Escherichia coli.

In summary, it was determined that E. coli was associated with sodium selenite and vitamin E deficiency.

4. Treatment. Add 100 ml of sodium selenite vitamin E needle (containing 0.5 mg vitamin E) to drinking water in the morning for 3 days, and add enough amount of compound vitamins to the feed to increase the immunity and growth rate of the chicken; In the afternoon, add Conic (main component of kanamycin sulfate) in drinking water at a dose of 150 kilograms per 100 grams of water for 4 consecutive days while using Coptis for detoxification. Four days later, the condition was controlled. The flock returned to normal one week.

5. Experience. 1 Regularly disinfecting the sheds and their surroundings, timely cleaning the sheds and maintaining good ventilation are effective measures to reduce the incidence of E. coli disease. In addition, adding a certain concentration of chlorine in the water to ensure the clean water, but also play a role in the prevention and treatment of E. coli. 2 Cut off the route of transmission in time, and the sick and dead chickens must do harmless treatment. Do not throw them in disorder. Diseased chickens should be treated in isolation and the contaminated environment of infected chickens should be thoroughly disinfected. 3 Because E. coli is susceptible to antimicrobial agents, it must be screened for sensitive drugs through drug susceptibility testing. 4 Preventive medications: The feeds cause selenium and vitamin E deficiency in the process of processing and storage. Therefore, 2 days old drinking water add sodium selenite vitamin E needles, 100 chickens can add 10 ml (containing 0.5 mg vitamin E). It can reduce selenium and vitamin B deficiency in broilers. It can also reduce the occurrence of ascites. Antibiotics are added to feed or drinking water to prevent the occurrence of E. coli.

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