Blood pressure monitoring technique for conscious free activity rats
From the perspective of whether the rat is anesthetized, the blood pressure can be divided into an awake state and an anesthesia state. Compared with anesthesia, rats have obvious differences in monitoring blood pressure during waking: (1) Overcoming the shortcomings of anesthesia, animals can move freely while monitoring blood pressure, and the monitored blood pressure is more accurate and reliable; (2) can be compared Monitoring blood pressure for a long time can achieve continuous monitoring of blood pressure for more than 24 hours; (3) more indicators can be measured: such as baroreceptor reflex sensitivity, blood pressure volatility, blood pressure day and night mode. Therefore, blood pressure monitoring in conscious rats plays an important role in cardiovascular pharmacology and cardiovascular pharmacology.
The device for monitoring blood pressure in a freely awake state of the rat includes a tail artery pressure measuring system,
Hydraulic monitoring system and implant remote pressure measuring system. Because the implanted remote pressure measuring system is expensive, the tail artery pressure measuring system is susceptible to the tail. Therefore, the hydraulic monitoring system is widely used in China.
In the state of conscious and free movement of rats, the use of hydraulic monitoring system to monitor blood pressure is technically demanding, and it is difficult for general laboratories to carry out related work. In order to better carry out this technology, we believe that the following technical points should be grasped:
(1) Rat anesthesia: It is best to use ketamine and diazepam combined anesthesia, the most method of this kind of hemp inhibits the baroreceptor reflex time; (2) catheter selection: soft and hard, the catheter is too hard Broken, and the chance of puncturing the blood vessels is too much; the catheter is too soft, the intubation is difficult, and the pressure recording is not accurate; (3) Intubation technique: the PE50 catheter is preferably used for the venous cannula, and the arterial cannula is connected to the PE50. For PE10 catheters, the cannula should be fixed near the inserted blood vessel, and the ligation should be tight; (4) the catheter connection must be tight; (5) the fluid path is guaranteed to be free of air bubbles; (6) the system is disabled for more than 3 days, and the catheter is placed Soak in physiological saline for use.
To truly carry out this technology, further information is needed, and more importantly, hands-on practice.
references:
Su Dingfeng, editor. Cardiovascular Pharmacology. Beijing: Science Press, 2001.
Zhang Hong, Su Dingfeng, Cheng Yong. Microcomputer monitoring and analysis system for conscious free animal hemodynamics. Chinese Journal of Physical Medicine. 1991, 13(4): 236-239
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