Nutritional rice noodles infant food supplement six kinds of aflatoxin detection solutions

Nutritional rice flour infant food supplement aflatoxin detection solution

With the emergence of social problems such as tainted milk powder, "stone dolls" and milk safety, the safety of traditional infant foods has been questioned by the whole society. Today, in the food safety of dairy products, the safety of infant food supplements that lacks attention is even more worrying. Recently, the quality of infant rice noodle products announced by the General Administration of Quality Supervision, Inspection and Quarantine showed a pass rate of only 58.3%! The safety of the foreign brands that parents are superstitious has also been broken. Recently, the baby food supplements of nine well-known European brands such as Nestle have also been found to have food safety problems that seriously harm the health of infants and young children.

Infant and childhood is the most important period of human growth and development, and the most vulnerable period. The safety of infant food can not be sloppy. From the 6 months of infants and young children, it is necessary to gradually add some foods other than breast milk to the infants. These foods are supplementary foods for infants and young children, referred to as infant food supplements. The materials of the complementary food are mostly rice, mainly noodles, followed by fruits and vegetables, such as common infant and young children's nutrition egg milk rice bran, nutritious rice noodles and so on. Today, the food industry is plagued by aflatoxins. These products must also attract the attention of society and enterprises. It is necessary for enterprises and national quality inspection departments to strengthen the detection and supervision of mycotoxins in infant food supplements to ensure infant food. Security.

As a domestic supplier of mycotoxin detection technology and product services, Tellabs Test Application Lab focuses on food safety incidents, especially on the safety of mycotoxins. The following solutions are proposed for infant food supplements. .

Immunoaffinity column + KRC photochemical post-column derivatization

1 Introduction
The PriboFast aflatoxin B+G+M immunoaffinity column (No. IAC-014-3 or AFT-A01) selectively adsorbs toxins from the sample solution for very targeted purification of the toxin sample and then HPLC/FLD+KRC post-column derivatization system can effectively determine the concentration of aflatoxin B1, B2, G1, G2, M1 and M2, and improve the accuracy and sensitivity of detection. PriboFast AF B+G+M immunoaffinity column is the national standard GB/T23212-2008 (measurement of aflatoxin B1, B2, G1, G2, M1, M2 in milk and milk powder by liquid chromatography-fluorescence detection method, Qinhuangdao inspection and quarantine The bureau has developed a product.

2. Characteristics of immunoaffinity column/KRC derivatization method • Strong specificity makes sample purification excellent;
• The use of immunoaffinity column operator can make sample purification fast and improve detection efficiency. • HPLC+FLD, KRC post-column derivatization, effectively improve detection sensitivity and achieve lower detection limit. • Derivatization is convenient, and photochemical derivatization does not require derivation. Reagents (usually derivatization is required after electrochemical column derivatization, the analysis cost is high, the operation is troublesome, and the instrument failure rate is high).
• High recovery rate of over 90%

3. Introduction to the procedure of immunoaffinity column method
1) Sample processing (infant rice noodle food supplement)

• Accurately weigh 25g of powdered sample and dissolve it in 125ml of methanol/water (70/30);

• Oscillate for 30 minutes (120 times/min) on the oscillator. It is also possible to mix using a magnetic rotor.

• Centrifuge all supernatants at 3000g for 5 minutes or filter;

• The clarified filtrate was dissolved in 0.01 M PBS solution (pH 7) at 1:10, passed through a glass fiber filter paper, and the filtrate was used.

2) Install the regenerative immunoaffinity column on the pump flow frame for column purification: after PriboFast aflatoxin B1B2G1G2M1M2 immunoaffinity column (IAC-014-3) activation, elution, elution three steps, elution The solution can be directly used for HPLC detection;
3) Determination: Direct injection of HPLC, directly recording and calculating the concentration of aflatoxin B1B2G1G2M1M2 after aflatoxin-specific chromatography column and KRC photochemical column derivatization fluorescence detector;

Pribofast KRC post-column derivatizer and HPLC parameters (in accordance with GB23212-2008)

PriboFast KRC-25 Post-column Photochemical Derivative Wavelength: 254 nm
HPLC-Column: Aflatoxin-specific column 250 x 4.6 mm; C-18
Mobile phase: water + methanol + acetonitrile 11 + 4 + 5
Flow rate: 1.0 mL/min Column temperature: 35 °C
Injection volume: 100μL
Fluorescence detector: λ-excitation wavelength: 355nm λ-emission wavelength: 430 nm

Liquid chromatograph detection configuration

High performance liquid chromatography with fluorescence detector
Pribofast KRC-25 post-column photochemical derivatization unit with 1ml reaction cell, nano tube 254nm
Aflatoxin-specific column C18 250 x 4.6 mm

In the figure, AFM2 M1 G2G1B2B1

Aflatoxin detection related product ordering information

Note: PriboLab is one of the leading providers of solutions for mycotoxin detection technology worldwide. With a strong R&D team and research on mycotoxin detection technology, it provides professional services for the global agricultural production, food processing and food, feed industry and other industries. Mycotoxin detection technology products and technical services. As the only partner of PriboLab in China, Beijing Tellabs actively provides high-quality mycotoxin detection technology and product services to domestic customers, providing domestic customers with suitable mycotoxin detection solutions to help domestic customers establish efficient and high-quality mycotoxin project testing. platform.