Can be divided into three categories, 4.5 can be seen from the experimental clustering diagram. Among them, the No. 31013 woody fragrance sample is one type, and the No. 411 soil wood fragrance is one kind, which is consistent with the experimental expectation, but there are some woody samples (the sample of 567151617) and Chuanmuxiang (the sample of 1289121418) are grouped together. Investigating the appearance of the woody samples, the cross-section color of No.31013 woody is deeper than other woody samples, and the smell is more intense. The true source of the sample No. 56717 is unpredictable, but the two wood samples (1516) of the clear source are clustered with Chuanmuxiang (121418). It is still to be studied experimentally to change the growth environment; it suggests that there are differences in the types and contents of volatile constituents from the woody flavor of Yunnan, and whether it can be used as a woody medicinal herb, and it is still necessary to combine component analysis and pharmacological effects.
Capillary column: HP 250.32 mm 0.25 μm 30 m carrier gas: N299.999% detector: hydrogen flame. RESULTS: Under the selected chromatographic conditions, Abstract: Objective To identify woody, Chuanmuxiang and Tumuxiang. Method: Gas chromatography was used. A gas chromatograph with stable and controllable medicinal materials was established; the application system clustering method can be divided into three categories.
Conclusion: It is feasible to identify woody, Chuanmuxiang and Tumuxiang by gas chromatography and systematic clustering.
The function is to relieve pain, and woody is a commonly used traditional Chinese medicine. It is used for chest pain, heavy after diarrhea, and the food is not lost. Chuanmuxiang is a habit, and its function is to relieve pain. It is used for chest pain, pharyngeal diarrhea, urgency and urgency, and two flank is not comfortable, hepatobiliary pain and other symptoms. Tumuxiang is less commonly used in traditional Chinese medicine, functioning spleen and stomach, regulating Qi Detoxification, pain relief and fetus, from the functional point of view, woody and Chuanmuxiang are closer, while the soil is different. The Chinese Pharmacopoeia 2000 edition stipulates that the woody scent should be the dried root of the AsterlandialappaDecn in the compositae; the genus Chuanmuxiang should be the dried root of the genus VladimiriasoulieiFranch.Ling or VladimiriasoulieiFranch.Lingvar.cinereaL. In the 2000 edition of the Chinese Pharmacopoeia, except for the determination of the content of the wood aroma lactone under the woody fragrance, the other two have not specified the content determination and only the identification item, and the Chuanmuxiang also contains the local woody volatile component. It is helpful to judge the appearance of the medicinal materials, and the identification of the woody and Chuanmuxiang in the proprietary Chinese medicines is difficult to judge by only one or two kinds of reference products. Therefore, the woody and Chuanmuxiang in the Chinese patent medicines The identification of Tumuxiang has the need for further research.
This is a method of using mathematical methods to classify a group of things by their "attributes." Cluster analysis as a quantitative method, cluster analysis in recent years (clusteringanalysi is increasingly used in the field of drug analysis. From the perspective of data analysis, a more accurate and detailed classification tool is given. Currently, there are also in the field of drug analysis. Applied.
Therefore, the medicinal materials and decoction pieces of the above three methods by gas chromatography are used as research objects, since the above three types of medicinal materials and decoction pieces contain volatile components. A systematic clustering study was carried out on the chromatograms in order to provide a new method for the identification of three woody fragrances, which provided a basis for the identification of three woody fragrances in Chinese patent medicines.
1 data and instruments
Sample: Woody (921-200104 Chuanmuxiang (1091-200001) Tumuxiang (1090-200001 or more of the reference medicines are provided by China National Institute for the Control of Pharmaceutical and Biological Products. The rest of the samples are provided by the Chinese Herbal Medicine Laboratory (both by the author according to China) The Pharmacopoeia 2000 edition and the "Regulations on the Finishing and Quality of Commonly Used Chinese Medicinal Materials", the second volume of the Southern Collaborative Group.
Instrument: Agilent 6890N gas chromatograph; hydrogen flame detector; carrier gas is high purity nitrogen (99.999% column is HP25 capillary column (0.32mm 0.25μm 30mAgilentChem ChemStation (A.08.03
Reagent: Methanol is analytically pure (batch Hangzhou Chemical Reagent Co., Ltd.).
2 experimental methods
2.1 chromatographic conditions
Split ratio 20:1 inlet temperature 200 ° C (sequential temperature rise: 70 ° C, split injection. 5 points) 10 ° C / min to 200 ° C, 200 ° C for 12 min injection volume is 1 μL
2.2 Preparation of test solution
Precisely add methanol 10mL at room temperature for 24h to take the supernatant as the test solution, accurately weigh the above samples (passing No. 2 sieve) about 0.5g in a 10mL volumetric flask. The chromatogram is obtained by injection.
2.3 GC chromatogram creation and calculation of relative area
There is a relative area value for each storage time (RA RA = A x100 / rA RA is the relative area value. Ax is the area of ​​a certain peak, according to the tailing peaks in the chromatogram of the test sample (tailingpeak is rare). The storage time of the chromatographic peaks is sorted. rA is the area of ​​the total baseline composed of the peak area. A = × σ × h = 2.507 σh = 1.064Wh / 2h > peak area. The storage time and relative area value of the test sample.
3 results
Systematic cluster analysis (using medianclust Euclidean square distance) analysis process was performed using SPSS10.0 statistical software.
4 discussion
The number of trays is 24.337min. The peak value is 436854. One injection can obtain more chromatographic peaks (a total of 50 peaks with different storage times in 18 samples are applied to the cluster analysis). 4.1 Experiment The GC method was used for chromatographic separation. Moreover, the storage time is relatively stable, and the gas chromatogram has certain stability and controllability.
Therefore, the total peak area is calculated as the peak area of ​​all chromatographic peaks at 5 min and 33 min. 4.2 The chromatogram of the test sample is removed from the solvent peak during data processing.
The results were maintained at 70 ° C for 5 min and then at 10 ° C / min to 200 ° C. In 4.3 experiments, different starting temperatures, different heating rates, and different endpoint temperatures were tested for chromatographic conditions. It is better to keep it at 200 ° C for 15 min, which can meet the experimental requirements.
There have been many reports, 4.4 on the identification of woody, Chuanmuxiang and Tumuxiang and the determination of the chemical components contained therein. Through the analysis of the volatile components of Muxiang, Chuanmuxiang and Tumuxiang, it is indicated that the requirements of identifying woody, Chuanmuxiang and Tumu can be satisfied by gas chromatography.
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