Microbial detection method

Some microorganisms are beneficial, and some microorganisms can cause a variety of strain-related diseases and infectious diseases, which will have adverse effects on human health; it will also cause food and raw materials to rot and deteriorate. In air or liquid, the selection of suitable test equipment plays an indelible role for the collection of microorganisms.

1.     Microbial detection method

( 1 ) Applied to liquid microbial detection - filter method

Connect the sterilized filter to the filter bottle, clamp the filter with sterile forceps, and place the filter on the bottom of the filter. Aspirate a certain amount of liquid sample or sample diluent into the filter, and open the vacuum pump for suction filtration. After all the sample passed through the filter, the filter edge was lightly washed at least twice with 20 mL - 30 ml of sterile physiological saline. Turn off the vacuum pump, open the filter, and remove the filter with sterile forceps. The filter was placed on a selective medium for cultivation. Generally, more filter devices are needed, and multiple filters are preferred, which reduces the number of tests, saves time, improves test accuracy, and reduces test errors.

( 2 ) Method for determining the total number of bacteria in the air applied to the gas

Our method developed according to the principle Anderson hit the air planktonic bacteria sampler, can effectively evaluate air quality. Feng Fumin et al., in the article "Comparison of three methods for detecting microorganisms in the air of hospitals", through a plate sedimentation method, a monitor method and a sampler method, respectively, a one-day continuous sampling of the hospital outpatient hall and the maternal and child ward ward respectively . The variance analysis and paired t- test were used for statistical processing. The results showed that the capture rates of the three sampling methods were significantly different . The sampler method was superior to the monitor method and better than the plate sedimentation method. The sampler method was used to monitor the highest rate of airborne bacteria capture. , it is a preferred method of sampling.

2.     Necessity of microbiological testing

(1) Food

Foods contain microbes that depend on growth for their microbes, so foods are contaminated with varying degrees of microbial contamination. Food quality and safety are related to the physical health, life safety and social economy of the people. For the safety of food, people's health and microbiological testing are extremely necessary.

( 2 ) Hospital

Hospital infections are closely related to microbes. Strengthening microbiological testing and monitoring in hospitals plays an important role in preventing and controlling nosocomial infections.

( 3 ) Air

Indoor air is an important means of transmission of microbial contamination. The comfortable indoor temperature, humidity and other environmental conditions as well as the relatively closed interior create conditions for the breeding of various harmful microorganisms. Only by effectively detecting the content of microorganisms can effective measures be taken to ensure that human health is not harmful to microorganisms.

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